Effects of a greens-based drink on blood epigenetics

A Greens-Based Crossover Trial to Improve Epigenetic Aging in Adults

Dr. Jordan Moon & Dr. Mike Roberts

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https://www.nih.gov/news-events/nih-research-matters/research-context-can-we-slow-aging

• Question: What percentage of participants showed a reduction in aging?
  • Over 60% (11 out of 19)
• Question: What was the avg. reduction (lowering) of aging among participants?
  • 10% reduction in DamAge:
  • DamAge tracks harmful changes caused by factors such as inflammation, pollution, stress, and poor lifestyle.
    • High DamAge = Faster aging, higher disease risk, shorter lifespan.
  • 12.8% increase in AdaptAge:
  • AdaptAge tracks helpful, protective changes your body makes to fight off aging.
  • AdaptAge = Better resilience, longer healthspan, and potentially longer life.

Executive Summary:

How FOG Lowers Biological Age

Biological age reflects how old your body really is, not just how many birthdays you’ve had. The lower your biological age, the healthier, more energetic, and longer-living you’re likely to be.

Field of Greens works by improving key dimensions of aging:

• DamAge (Damage Age) – the biological wear and tear your body accumulates
• AdaptAge (Adaptation Age) – your body’s ability to bounce back and protect itself

Together, improving DamAge and AdaptAge can reliably reduce biological age and help people feel and function younger.

The Science Made Simple

Recent research (Nat Aging . 2024 February ; 4(2): 231–246. doi:10.1038/s43587-023-00557-0.) has discovered a new way to measure aging by utilizing DNA methylation to track chemical changes that control how your genes behave. These changes can fall into two categories:

• DamAge tracks harmful changes caused by things like inflammation, pollution, stress, and poor lifestyle.
  • High DamAge = Faster aging, higher disease risk, shorter lifespan.
• AdaptAge tracks helpful, protective changes your body makes to fight off aging.
  • High AdaptAge = Better resilience, longer healthspan, and potentially longer life.

Unlike traditional aging tests, these measures are causally linked to real health outcomes, including disease risk and mortality.

What Field of Greens Does

FOG has been shown to reduce Biological Age in two powerful ways:

1. Reduces DamAge

Lowers the biological "damage load" from oxidative stress, inflammation, and cellular wear, helping slow down the rate at which cells and organs deteriorate.

2. Boosts AdaptAge

Enhances the body’s own defense mechanisms, such as detoxification, DNA repair, immune balance, and energy metabolism, supercharging your body's natural resilience, much like how elite athletes recover faster and stay healthier for longer.

Takeaways

Field of Greens targets the root causes of aging by reducing cellular damage and enhancing biological resilience, clinically linked to lower biological age, better health span, and longer life.”- Dr. Jordan Moon

• Field of Greens is a science-backed longevity solution that
• stands apart from generic anti-aging products.
• Field of Greens is not just helping people live longer, but helping them live younger.

Auburn clinical trial recommendation and support for claims

Authors: Jordan Moon, PhD, Mike Roberts, PhD (in consultation with Drew Fruge, PhD, RD and co-PI of trial)

Synopsis: These claims are based on the interim report provided to the sponsor’s liaison in April 2025. Anything in quotations and blue [see “Sample language” here] is a technically accurate claim supported by the Auburn Research Team.

Primary outcome: Immune cell epiAge

Simple claim: “Field of Greens positively impacts immune cell epiAge.”

Expanded claim: "In a clinical study involving middle-aged, overweight adults, Field of Greens positively altered two key immune cell-specific markers of biological age – specifically a 7.8% increase in “AdaptAge” and a 2.2-unit reduction in “DamAge”. These data suggest Field of Greens may help maintain healthy aging processes at the cellular level." ***

Expanded discussion: Tissue DNA methylation patterns (aka, DNA methylation epiAge clocks) have been used as surrogates of biological aging. However, severe limitations exist with older epiAge clock algorithms given that immune cells are commonly used in an attempt to define whole-body biological age (https://www.aging- us.com/article/206184/text). To combat this limitation, researchers validated two newer -generation biological age clocks in 2024 specific to immune cells (termed “AdaptAge” and “DamAge”) https://pmc.ncbi.nlm.nih.gov/articles/PMC11070280/. Notably, this publication included Steve Horvath, who is considered by most to be the pioneer of the biological aging field. Unlike first generation DNA methylation clocks that didn’t leverage artificial intelligence and machine learning, the authors set out to use these technologies to refine the AdaptAge and DamAge clocks so that (in their words) “AdaptAge contains only the CpG sites that capture protective effects against aging” and “DamAge contains only the CpG sites that exhibit damaging effects, which may be considered as a biomarker of age-related damage”. Importantly, a positive increase in AdaptAge is protective against aging, and a reduction in DamAge is protective against aging. The findings that Field of Greens supplementation elicited positive increases in AdaptAge and DamAge suggest that Field of Greens helps maintain healthy aging processes at the cellular level.***

***technical note: This statement is based on statistical analysis where AdaptAge increased 7.8% during the 30- day Greens supplementation (63% of participants showed a “health-associated” increase in this marker), whereas

AdaptAge decreased 5.0% during the 30-day no supplementation period. Statistical outcomes for AdaptAge were as follows on percentage change scores:

• a moderate effect: Cohen’s d = 0.566
• dependent samples t-test (two-tailed): p=0.223
• dependent samples t-test (one-tailed): p=0.111

Statistical analysis of DamAge showed a -0.9% change during the 30-day Greens supplementation (60% of participants showed a “health-associated” decrease in this marker), whereas DamAge increased 10.9% during the 30-day no supplementation period. Statistical outcomes for DamAge were as follows on percentage raw scores:

• a moderate effect: Cohen’s d = 0.684
• dependent samples t-test (two-tailed): p=0.181
• dependent samples t-test (one-tailed): p=0.091

As well, participant #1 was removed from the DamAge analysis given that his V3 values were approached lower quartile cutoffs for outlier removal, and as a result, his percentage change in DamAge yielded a >2SD value.

Subject Characteristics

Adherence to Greens consumption

All participants completed the study, with no withdrawals. Adherence to the supplement regimen was strong, as all participants regularly responded to reminders. They appreciated the daily notifications and communicated if they missed a dose, which occurred infrequently.

Overall, adherence to the greens supplement was high, with participants demonstrating strong engagement and compliance throughout the study.

Claims based on Initial Analysis May 2025

1. Improved Cellular Health: Epigenetic changes in immune cells may favor healthier function
2. Enhanced Energy Levels: Increased energy was one of the most reported benefits.
3. Digestive Improvements: Almost half of the subjects reported improved digestion and bowel regularity and reduced bloating as well as better stool consistency
4. Mental Health Benefits: 1/3 of the subjects reported improvements in mental clarity, focus, and mood when taking the
5. Physical Health Improvements: Some participants observed unexpected benefits, such as healthier nails, reduced joint pain, or better skin health
6. Results you can feel: More than half of the subjects reported perceived general health and wellness benefits
7. Metabolic Health and Safety: No adverse outcomes for liver and kidney function as well as no abnormal changes in hematological or clinical chemistry/metabolic markers